Initial sample prep

Initial sample prep is not described here, but is according to the method of: Eide, D.J., Clark., S., Nair, T.M., Gehl, M., Gribskov, G., Guerinot, M.L., and Harper, J.F., 2005, Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae. Genome Biology, v. 6, R77. The resulting solution from this initial sample prep is ~1 ml in 15% HNO3.

Dish washing

Low Cu and Zn concentrations are to be analyzed. Experience has shown that our usual cheap 10 ml polypropylene test tubes have minor Cu and Zn contamination. This can be removed by soaking for several hours in 1% HNO3.

Diluting solution

To a 1000 ml clean plastic bottle add 7 ml of high-purity HNO3 and 10 μL of the Ga 1000 μg/ml single element solution as an internal standard (0.5% HNO3, 10 ppb Ga).

Stock standard solution

To a 100 ml volumetric flask add 3.5 ml of high-purity HNO3.  Add to the flask the amounts of the 1000 μg/ml single element solutions shown in the yellow column below.  Dilute to volume and put into a clean storage bottle.

 

  ml of each 1000 μg/ml element solution per 100 ml of stock standard Concentration in the stock standard, ppb Concentration in the final standard, ppb (2.5 ml of the stock standard per 50 ml)
Mn 0.002 20 1
Ca 0.2 2000 100
Fe 0.01 100 5
Ni 0.01 100 5
Mg 0.2 2000 100
P 2 20000 1000
Zn 0.1 1000 50
Co 0.01 100 5
Cu 0.01 100 5
Na 0.2 2000 100
K 0.2 2000 100

Dissolution procedure Part 2

  1. Using 10 ml of the diluting solution, transfer the samples into the cleaned 10 ml test tubes.
  2. For the blank, add 50 ml of diluting solution to the 50 ml blank test tube.
  3. For the standard, add 50 ml of diluting solution to the 50 ml standard test tube.  Add 2.5 ml of the stock standard solution.

Kurt Hollocher

Geology Department

Union College

Schenectady, NY  12308

U.S.A.