Undergraduate Research

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Until the late 19th century, Union students were not supposed to leave campus without a faculty chaperone. Today students are encouraged to take in the many off-campus opportunities by using a "swipe card" at local businesses.

Epitope FLAG-tagging the Schizophyllum commune sugar transporter Sts1 - Michael Gallagher

Hometown: Unionville, Connecticut
Class Year: 2008
Major: Biological Sciences Minor: German
Research Advisor: Professor JS Horton

Project Description:

This summer my goals were to tag a protein, called Sts1, in the wood mushroom Schizophyllum commune, with a specific peptide sequence. I did this by manipulating S. commune genes with microbiological techniques. Since genes hold the code for all the proteins produced in a cell, if I made the right changes in the DNA I could have the cell make the protein I wanted. This peptide I added (which is basically a small protein) allows antibodies to bind to it, which ultimately means antibodies can bind to the target protein. Some antibodies fluoresce under certain wavelengths of light, so if I have a tagged Sts1 protein, I could tell where Sts1 is in the S. commune cells when looking under a microscope.

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Home News Research Opportunities Summer Research Showcasing at Union Showcasing Nationally Internal Education Funds Important Deadlines Did U Know? Until the late 19th century, Union students were not supposed to leave campus without a faculty chaperone. Today students are encouraged to take in the many off-campus opportunities by using a "swipe card" at local businesses.	Epitope FLAG-tagging the Schizophyllum commune sugar transporter Sts1 - Michael Gallagher Hometown: Unionville, Connecticut Class Year: 2008 Major: Biological Sciences Minor: German Research Advisor: Professor JS Horton Project Description: This summer my goals were to tag a protein, called Sts1, in the wood mushroom Schizophyllum commune, with a specific peptide sequence. I did this by manipulating S. commune genes with microbiological techniques. Since genes hold the code for all the proteins produced in a cell, if I made the right changes in the DNA I could have the cell make the protein I wanted. This peptide I added (which is basically a small protein) allows antibodies to bind to it, which ultimately means antibodies can bind to the target protein. Some antibodies fluoresce under certain wavelengths of light, so if I have a tagged Sts1 protein, I could tell where Sts1 is in the S. commune cells when looking under a microscope.
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